World Congress on Thyroid Cancer 4.0
July 29 – August 1, 2021
EP36 – Panel of microRNAs expressed in FNAB material of thyroid nodules and its application as molecular test
Ferraz, Carolina1; Bittar, Gustavo1; Paschke, Ralf2; Eszlinger, Markus3; Padovani, Rosália1,4
1 Endocrinology Unit, Internal Medicine Department of Santa Casa de Sao Paulo, School of Medical Sciences, Sao Paulo, Brazil
2 Departments of Medicine, Oncology, and Biochemistry and Molecular Biology, & Arnie Charbonneau Cancer Institute, Cumming School of Medicine, University of Calgary, Calgary, Canada
3 Department of Oncology & Arnie Charbonneau Cancer Institute, Cumming School of Medicine, University of Calgary, Calgary, Canada
4 Nuclear Medicine Service of Santa Casa de Sao Paulo, School of Medical Sciences, Sao Paulo, Brazil
Background: Thyroid cancer (TC) is the most common endocrine neoplasm and fine needle aspiration biopsy (FNAB) is the gold standard for evaluating thyroid nodules1. However, 25-30% of all FNABs cannot distinguish between malignant and benign lesions, being therefore classified as “indeterminate”1. Molecular markers have been shown to play an important role in solving this problem2. MicroRNAs (miR) may act as possible molecular markers3.
Methods: Through literature search and prior data from our cooperative group4, 6 miRs were chosen to compose a panel and their expression profiles were evaluated in 11 FNAB samples (2 benign, 3 AUS/FLUS, 2 folicular neoplasm and 4 papilary carcinoma) by RT-qPCR in order to distinguish benign from malignant samples. Final histology was used to validate the panel.
Results: The final panel was composed of miR-146b,-221,-222,-484,-139-5p, and -145-5p. MiR-146b showed a high sensitivity (80%) and specificity (75%) to differentiate malignant from benign nodules. Other miRs, when evaluated separately, did not present a significant value for differentiating samples. The 2 benign FNAB samples showed an occult microcarcinoma at final histology and showed a significant miR-146b overexpression.
Discussion/Conclusion: In our preliminary analysis, previous data with regard to the role of miR-146b as molecular marker were confirmed5. Although the expression of the other miRs did not present differential expression in malignant tissues, an evaluation of a larger number of samples could improve statistical analysis and clarify their role as molecular markers in TC. Interestingly, miR-146b showed an overexpression in occult microcarcinomas in 2 benign FNAB samples.
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- Haugen BR, et al. The American Thyroid Association Guidelines Task Force on Thyroid Nodules and Differentiated Thyroid Cancer. Thyroid 2016. Volume 26, Number 1.
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